pPerturb - Thermodynamics

pPerturb

Extracting Energetic Couplings and Destabilization Thermodynamics via Perturbations

Ionic Strength (M)

pH

WT Melting Temp. (K)

Entropic Cost

(Uncheck if experimental Tm is not available for WT)

Temperature (K)

S U B M I T

1ubq - M1

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RECENTER

Residue folding probabilities and local stability profiles are predicted at this temperature. (Accepted range - 278 K < T < Tm)

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Ionic Strength from 0.01 M to 0.2 M

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pH value should be 5 or 7

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Provide experimental Tm of the wild type, between 283K and 373K

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Mutate Residue

to

Provide residue number as given in the uploaded PDB file

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Select entropic cost from the drop down list

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Protein Biophysics Lab

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Last Updated: September 25, 2019